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Peptide Sequence
Enter Sequence in Capital letters (B, J, O, X, Z not allowed) except:
| m - Met-ox | h - Homoserine lactone | U - Selenocysteine |
| s, t, y - Phosphorylated S, T, Y | d, e, f, g, h, i, j, k, l - user specified amino acids |>
Modified amino acids may be entered using PSI notation - eg. M(Oxidation), S(Phospho)
[-] Click + to see list of available PSI modifications (enter exactly as shown)
An amino acid can also be followed by an exact mass - eg. P(-27.9949) or N(0.9840)
Modifications may be added together - eg. X(MappingN+Deamidated)
Modified N and C termini must be selected from the menus

N Term Sequence C Term
[-] Additional Sequences
User Specified AA Elem Comp (d)
User Specified AA Elem Comp (e)
User Specified AA Elem Comp (f)
User Specified AA Elem Comp (g)
User Specified AA Elem Comp (h)
User Specified AA Elem Comp (i)
User Specified AA Elem Comp (j)
User Specified AA Elem Comp (k)
User Specified AA Elem Comp (l)
Use instrument specific defaults to override ion types below Glyco
AA
Composition
N-term
Sequence
C-term
Sequence
Internal
Fragment
Ladder
Sequencing

i

m

a

b

c+2

c+1

c

c-1

x

y

Y

z

z+1

z+2

z+3

Internal

N-term

C-term
Satellite Sequence
(side-chain loss)
Neutral-loss
Sequence
Peeling
Sequence

d

v

w

-H2O
S, T, E, D

-NH3
R, K, Q, N

-H3PO4
(S, T, Y + PO4)

-SOCH4
(M + Ox)

b+H2O
R, H, K
Max Charge Max Losses Max Internal Len Multi Z Internal
Display Graph Output Hits to file Name
Masses are Frag Tol
Filter m/z to z to Masses On
Instrument Data Format
Data Paste Area