Conference: ASMS 1998
Session Code: 	WPD 	Slot: 	175
Title: 	Automated Protein Identification by MALDI-MS
Authors: 	*Clauser, Karl; Tang, Chris; Rosenberg, Stan; Baker, Peter; Hsieh, Frank;
Institutes: 	
Abstract: 	Delayed-extraction and reflectors have improved the mass accuracy obtainable
on MALDI-TOF instruments almost to the point where elemental composition can be routinely
determined for each component in a peptide mixture. Hence, when peptides are derived from
digestion of 1-3 proteins in a mixture and the sequence of those proteins exist in a genome
database, peptide mass fingerprinting allows high-confidence identification of the proteins.
We have developed an integrated system for automating the portions of this process following
protein isolation. The following components will be described: digestion, de-salting, MALDI
target spotting, spectral acquisition, spectral processing, and database searching.