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Peptide Sequence
Enter Sequence in Capital letters (B, J, O, X, Z not allowed) except:
| m - Met-ox | h - Homoserine lactone | U - Selenocysteine |
| s, t, y - Phosphorylated S, T, Y | d, e, f, g, h, i, j, k, l - user specified amino acids |
Modified amino acids may be entered using PSI notation - eg. M(Oxidation), S(Phospho)
[-] Click + to see list of available PSI modifications (enter exactly as shown)
An amino acid can also be followed by an exact mass - eg. P(-27.9949) or N(0.9840)
Modifications may be added together - eg. X(MappingN+Deamidated)
Modified N and C termini must be selected from the menus

N Term Sequence C Term
[-] Additional Sequences
[-] User Specified AA Elem Comp
Use instrument specific defaults to override ion types below Glyco All Glyco
AA
Composition
N-term
Sequence
C-term
Sequence
Internal
Fragment
Ladder
Sequencing

i

m

a

b

c+2

c+1

c

c-1

x

y

Y

z

z+1

z+2

z+3

Internal

N-term

C-term
Satellite Sequence
(side-chain loss)
Neutral-loss
Sequence
Peeling
Sequence

d

v

w

-H2O
S, T, E, D

-NH3
R, K, Q, N

-H3PO4
(S, T, Y + PO4)

-SOCH4
(M + Ox)

b+H2O
R, H, K
Max Charge Max Losses Max Internal Len Multi Z Internal
Display Graph Output Hits to file Name
Masses are Frag Tol
Filter m/z to z to Masses On
Instrument Data Format
Data Paste Area